14 Primer design

Designing PCR primers for target regions using Primer3 and eprimer3.

14.1 Primer design for genes with IDs as input

  1. Essential parameters: Only Species, Input type, Sequence type, and Data input are needed for basic usages.
  2. Four optional parameters for controlling primer designs are supplied with detailed explanations.

Simply, users just need to select each parameter sequentially as labeled in the figure (or simply just click the Demo1, and Demo2 buttons).

A screenshot of the one type of selection for Primer design.

Figure 14.1: A screenshot of the one type of selection for Primer design.

Clicking Submit, after several seconds, the primer results would be listed below.

Tables of forward primers (FP) and reverse primers (RP) with related attributes.

Figure 14.2: Tables of forward primers (FP) and reverse primers (RP) with related attributes.